Comparison of methods for the identification of coagulase-negative staphylococci.

نویسندگان

  • Maria de Lourdes R S Cunha
  • Yuri K Sinzato
  • Liciana V A Silveira
چکیده

Coagulase-negative staphylococci (CNS) species identification is still difficult for most clinical laboratories. The scheme proposed by Kloos and Schleifer and modified by Bannerman is the reference method used for the identification of staphylococcal species and subspecies; however, this method is relatively laborious for routine use since it requires the utilization of a large number of biochemical tests. The objective of the present study was to compare four methods, i.e., the reference method, the API Staph system (bioMérieux) and two methods modified from the reference method in our laboratory (simplified method and disk method), in the identification of 100 CNS strains. Compared to the reference method, the simplified method and disk method correctly identified 100 and 99% of the CNS species, respectively, while this rate was 84% for the API Staph system. Inaccurate identification by the API Staph method was observed for Staphylococcus epidermidis (2.2%), S. hominis (25%), S. haemolyticus (37.5%), and S. warneri (47.1%). The simplified method using the simple identification scheme proposed in the present study was found to be efficient for all strains tested, with 100% sensitivity and specificity and proved to be available alternative for the identification of staphylococci, offering, higher reliability and lower cost than the currently available commercial systems. This method would be very useful in clinical microbiology laboratory, especially in places with limited resources.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Comparison of Disc Diffusion and E test methods for Antimicrobial Susceptibility Testing Of Vancomycin in Coagulase Negative Staphylococcus Isolated from Blood Culture

  Background and Objective: Reliable methods of detection of antimicrobial resistance are of paramount importance in the treatment and management of infections caused by coagulase negative staphylococci (CoNS). The objective of the present study was to compare and evaluate the performance of disc diffusion and E test (Epsilometer test) methods for antimicrobial testing of coagulase ne...

متن کامل

مقایسه روش تعیین حساسیت دیسک دیفیوژن و واکنش زنجیره ای پلیمراز برای شناسایی استافیلوکوکوس مقاوم به متی سیلین

Background: Staphylococci as a micro-organism, has the most importance to cause nosocomial infections, particularly in patients with indwelling catheters or other medical devices. Unfortunately 90% of Staphylococci isolated from the nosocomial infections are resistant to methicillin, and methicillin resistance strains are also resistant to a wide range of antimicrobial drugs, therefore detectin...

متن کامل

Prevalence and antimicrobial susceptibilities of coagulasenegative Staphylococci isolated from mammary secretions in pregnant Holstein heifers

Mammary secretions of 913 quarters from 229 pregnant Holstein heifers were obtained aseptically to determine the prevalence of coagulase-negative Staphylococci isolated during periparturient period. Quarter samples were collected 14 to 10 days before expected parturition. Analysis of culture data indicated that 78.5% (717/913) of samples were negative, 20.5% (187/913) contained coagulase-negati...

متن کامل

Isolation, Identification, and Profile of Antibiotic Resistance of Bacteria in Patients with Cancer

Background: Blood stream infection is one of the main causes of morbidity and mortality in patients with cancer. The purpose of this study was to evaluate the prevalence of aerobic and anaerobic-bacteremia in hospital inpatients with cancer and to determine the antibiotic resistance profile in isolated organisms in Kerman, southeast Iran. Methods: Total of 240 blood cultures from 136 patients w...

متن کامل

Evaluation of the antibiotic susceptibility of Coagulase Negative Staphylococci in children

Ghotaslou R1, Gharashi Z2, Pour-asl M3 1. Assistant professor, Department of pathobiology, Faculty of medicine, Tabriz University of medical sciences 2. Associate professor, Department of pediatrics, Tabriz University of medical sciences 3. Instructor, Department of pathobiology, Faculty of health, Tabriz University of medical sciences Abstract Background: The most common microorgani...

متن کامل

بررسی اثر سینرژیسمی آنتی بیوتیک‌های وانکومایسین و سفیپیم بر روی استافیلوکوک‌های کواگولاز مثبت و منفی مقاوم به متی سیلین

Background and Aim: Although vancomycine is used for the treatment of methicillin resistant Staphylococci, but vancomycine alone might not be effective for the treatment of methicillin resistant Staphylococci in compromised host. In the same time antimicrobial activity of cefepim on gram positive bacteria especially Staphylococci is well known. In this study the synergism effect of cefepim and ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Memorias do Instituto Oswaldo Cruz

دوره 99 8  شماره 

صفحات  -

تاریخ انتشار 2004